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Species- and group-specific PCR primers were developed to study predation on pest and nonpest invertebrate species by generalist carabid predators in agroecosystems. To ensure the amplification of degraded DNA in predator gut samples, amplicons were designed to be less than 300 bp. Specificity of primers was assessed by cross-amplification against a panel of target and nontarget invertebrate species. The new primers were combined with previously published primers for slugs and collembolla in multiplex reactions to simultaneously screen each predator for the presence of multiple prey. All prey species were detected in a screen of the gut contents of field-caught predators.

Original publication

DOI

10.1111/j.1755-0998.2010.02913.x

Type

Journal article

Journal

Mol Ecol Resour

Publication Date

03/2011

Volume

11

Pages

370 - 373

Keywords

Animals, DNA Primers, Ecosystem, Gastrointestinal Contents, Insecta, Predatory Behavior, Species Specificity